Carrot cells cell growth condition

In order to detect the effect of conditioning on growth, the stimulation of multiplication on carrot cell low-density cultures was utilized as bioassay. Our initial approach has been simply to repeat, with minor modification, the experiments of Street (5) and we confirmed that a CF is a molecule of low molecular weight that can diffuse through a dialysis membrane. 

Table 8. Plating efficiency of carrot cells at different cell densities in the presence of conditioning carrot callus. After 20 days of growth, the glass rings containing callus were removed and the plating efficiency, under and outside the rings, were determined. (Reproduced with permission from reference 20. Copyright 1989 Elsevier.)...

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