Carob galactomannan

Assay and Purification of p-Mannanases. p-Mannanase can be specifically measured in crude enzyme mixtures with a dye-labelled carob galactomannan substrate (6), This substrate has a galactose content high enough (23%) to impart solubility to the substrate, but sufficiently low as not to interfere with the action of p-mannanases on the D-mannan backbone. The soluble dye-labelled substrate is incubated with enzyme preparation under controlled conditions and the reaction is terminated and unreacted dyed polysaccharide precipitated by ethanol addition. 

Table I. Oligosaccharides produced on hydrolysis of carob galactomannan by p-mannanases...

In hot-water-soluble, carob galactomannan, the relatively high level of couplets of neighboring D-galactosyl groups would reflect a capacity for a repeat substitution on the opposite side of the mannan chain, once the steric hindrance involved in formation of the enzyme-substrate complex has been... 

Fio. 3.—Fractions of the D-Mannan Chain of Hot-water-soluble Carob Galactomannan (18% Content of D-Galactose) that Occur as Unsubstituted and Substituted D-Mannosyl... 

A way of reconciling the observed proportions of mannobi-, tri-, and tetra-oses and heterosaccharides,16113 released by guar-seed jS-D-mannanase (from hot-water-soluble carob galactomannan), with this model, would be if the enzyme preferentially hydrolyzes appropriate, unsubstituted d-mannosyl residues (as in 31) then, it would only be able to hydrolyze on the reducing side of a substituted D-mannose (as in 32) if there were a... 

Turquois, T., Rochas, C., and Taravel, F. R. (1992). Rheological studies of synergistic kappa carrageenan-carob galactomannan gels. Carbohydr. Polym. 17 263-268. 

Turquois, T, Taravel, F. R, and Rochas, C. 1993. Synergy of the agarose-carob galactomannan blend inferred from mnr and rheological studies. Carbohydr. Res. 238 27-38. 

Carob galactomannan. See Galactomannan Carob gum. See Locust bean (Ceratonia siliqua) gum... 

CAS 11078-30-1 EINECS/ELINCS 234-299-6 Synonyms Carob galactomannan D-Galacto-D-mannan... 

McCleary B.V., Amado R., Waibel R., Neukon H. (1981). Effect of galactose content on the solution and interaction properties of guar and carob galactomannans. Carbohydrate Research, 92, 269-285. 

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