Carbohydrate oxidase

Carbohydrate isomerases Carbohydrate oxidases Carboxypeptidase A Carboxypeptidase B... 

Table whether or not they are enzymically active. The following details of immobilized glycoside and polysaccharide hydrolases and carbohydrate oxidases are worthy of note. 

Carbohydrate oxidase (IridofAycut flaeeidum) Unknown Aldobionic acid -f Hrf), 34... 

EC 3.2.1 glycosidases, EC 4.2.2 lyases, EC 3.1.1 esterases, EC 1.11.1 peroxidases, EC 1.1.3 carbohydrate oxidases, EC 1.10.3 phenol oxidase, and other EC classes, according to their main reactions. Each class and subclass has shared primary enzyme substrates, a feature that may facilitate enzyme selections for fargefed biomass materials. 

Another method of forming aldehyde groups on carbohydrates and glycoproteins involves the use of specific sugar oxidases. These enzymes only affect the monosaccharide they are specific toward,... 

Enzymic methods are usually very specific and sensitive. Unfortunately the only methods in the literature for carbohydrates are all for glucose. Hicks and Carey [163] reported such a method, with a fluorometric final measurement, which was down to 3 x 10 8 M. Andrews and Williams [ 164] used a preconcentration step, sorption onto charcoal, elution, and a final determination with glucose oxidase. 

Mavicyanin (Mj = 18,000) is obtained from green squash (Cucurbito pepo medullosa), where it occurs alongside ascorbate oxidase [64]. It has a peak at 600 nm (e 5000 M cm and reduction potential of 285 mV. Further studies on this and the mung bean and rice bran proteins [65, 66] would be of interest. All the above type 1 Cu proteins have an intense blue color and characteristic narrow hyperfine EPR spectrum for the Cu(II) state. Table 3 summarizes the properties of those most studied. There is some variation in reduction potential and position of the main visible absorbance peak. In the case of azurin, for example, the latter is shifted from 597 to 625 nm. Stellacyanin has no methionine and the identity of the fourth ligand is therefore different [75]. The possibility that this is the 0(amide) of Gln97 has been suggested [63b]. It now seems unlikely that the disulfide is involved in coordination. Stellacyanin has 107 amino acids, with carbohydrate attached at three points giving a 40% contribution to the M, of 20,000 [75]. 

A liquid membrane bioreactor was developed as a means of encapsulation for a multi-enzyme system incorporating an oxidation and carbohydrate cleavage, demonstrated using a-glucosidase and glucose oxidase in the conversion of maltose to gluconic acid ... 

---