Carbohydrate affinity screening

The majority of reports have used electrospray ionization mass spectroscopy (ESI-MS) as an analytical detection method because of its sensitivity and the soft namre of its ionization procedure, which generally only leads to the detection of the molecular ions of the positive library members. Many separation techniques have been coupled to ESI-MS, including affinity chromatography (49), size exclusion chromatography (50, 51), gel filtration (52), affinity capillary electrophoresis (53-58), capillary isoelectric focusing (59), immunoaffinity ultrafiltration (60), and immunoaffinity extraction (61). ESI-MS has also been used alone (62) to screen a small carbohydrate library. Other examples reported alternative analytical techniques such as MALDI MS, either alone (63, 64) or in conjunction with size exclusion methods (65), or HPLC coupled with immunoaffinity deletion (66). 

Nakajima, K., Oda, Y., Kinoshita, M. andKakehi, K. Capillary affinity electrophoresis for the screening of post-translational modification of proteins with carbohydrates. J Proteome Res, 2, 81, 2003. 

Nakajima, K. et al. Screening method of carbohydrate-binding proteins in biological sources by capillary affinity electrophoresis and its apphcation to determination of Tulipa gesneriana agglutinin in tulip bulbs. Glycobiology, 14, 793, 2004. 

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