Carbamates pesticide hydrolysis, 300, Table

Table VI. Base Hydrolysis Rate Constants of Carbamate Pesticides and Metabolites at 15°C...

Carbamate pesticides are best analyzed by HPLC using postcolumn deriva-tization technique. Some common carbamate pesticides are listed in Table 2.19.1. Compounds are separated on a C-18 analytical column and then hydrolyzed with 0.05 N sodium hydroxide. Hydrolysis converts the carbamates to their methyl amines which are then reacted with o-phthalaldehyde and 2-mercaptoethanol to form highly fluorescent derivatives. The derivatives are detected by a fluorescence detector. o-Phthaladehyde reaction solution is prepared by mixing a 10-mL aliquot of 1% o-phalaldehyde solution in methanol to 10 mL of acetonitrile containing 100 pL of 2-mercaptoethanol and then diluting to 1 L with 0.05 N sodium borate solution. 

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