Capillary zone electrophoresis theory

Righetti, P.G., Gelfi, C., Perego, M., Stoyanov, A.V., and Bossi, A., Capillary zone electrophoresis of oligonucleotides and peptides in isolectric buffers theory and methodology, Electrophoresis, 18, 2145, 1997. 

The theories describing micellar electrokinetic chromatography (MEKC), capillary zone electrophoresis (CZE), and capillary gel electrophoresis (CGE) separations of small molecules and biopolymers are described in other chapters of this book and will not be discussed here. Here, we will briefly touch upon the theoretical aspects of the electrophoretic mobility of organelles, foregoing an in depth discussion of electrokinetic theory that can be found elsewhere in original publications and comprehensive reviews. The electrophoretic mobility (/ue) is defined as... 

Lukacs, K. Theory, instrumentation, and applications of capillary zone electrophoresis. PhD Dissertation, University of North Carolina, Chapel Hill, 1983. 

Capillary zone electrophoresis (CZE) is the most commonly used separation mode in CE microchip due to its versatility, ease of operation, and separation power. This electrophoretic mode is based on the theory and mechanism of action directly transferred from the conventional CE. In CZE the channel contains only a buffered medium and the electrophoretic mobilities of the analytes depend on their charge/mass ratio, acting as separation mechanism. These different velocities make possible to separate cations and anions if the EOF is strong, in order to lead anionic substances to the detector located usually at the cathode. It has the limitation of being useful only for separation of charged species. 

Kasicka, V., Pruslk, Z., Sazelova, P., Jiracek, J. and Barth, T., Theory of the correlation between capillary and free-flow zone electrophoresis and its use for the conversion of analytical capillary separations to continuous free-flow preparative processes. Application to analysis and preparation of fragments of insulin, ]. Chromatogr. A, 796, 211, 1998. 

Hjerten, S., Zone broadening in electrophoresis with special reference to the high-performance electrophoresis in capillaries An interplay between theory and practice. Electrophoresis, 11,665,1990. 

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