Capillary temperature band broadening

The electrolyte composition and pH should not be too detrimental. Aspects such as pressure, temperature, and injection technique may have an influence. Concerning the interface, the sheath flow should be optimized with respect to composition, pH, and flow rate. Furthermore, the positions of the fused-silica capillary as well as the API probe need to be carefully optimized. The mixing volume at the tip of the capillary must be kept at a minimum to avoid postcolumn band broadening caused by diffusion. 

Separations should initially be attempted with the capillary thermostatted at close to ambient temperature. The capillary temperature can be increased on most commercial CE units to as high as 60 C without substantially increasing current with most buffers. When this is done using the same applied voltage, decreased buffer viscosity leads to an increase in analyte electrophoretic mobility, thus, decreasing separation times. Also, it is important to note that, when sample introduction is hydrostatic (same pressure/vacuum and time), increased capillary temperature will lead to an increase in the injected sample volume as a result of decreased buffer viscosity. " Sensitivity may not necessarily be increased. However, Undesirable effects include concurrent changes in buffer pH, band broadening due to increased diffusion, and possible thermal denaturation of the sample. 

Xuan X, Li D (2005) Band broadening in capillary zone electrophoresis with axial temperature gradients. Electrophoresis 26 166-175... 

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