Capillary electrophoresis fingerprint

Figure 15.10 presents the two-dimensional capillary electrophoresis fingerprint of a single MCF-7 breast cancer cell. This cellhad been fixed in 70% ethanol before analysis. 

Hu, S., Zhang, L., Newitt, R., Aebersold, R., Kraly, J.R., Jones, M. andDovichi, N.J. (2003b) Identification of proteins in single-cell capillary electrophoresis fingerprints based on comigration with standard proteins. Anal. Chem. 75,3502-3505. 

DEVELOPMENT OF CAPILLARY ELECTROPHORESIS FINGERPRINTS AND MULTIVARIATE STATISTICS FOR THE DIFFERENTIATION OF OPIUM AND POPPY STRAW SAMPLES... 

The average DS as determined by these methods provides only the simplest characterization of these derivatives, and further analysis was necessary to characterize the mixture of SBE bands of different levels of substitution. Due to the presence of the anionic sulfonate substituent, it is possible to use capillary electrophoresis (23), to separate the SBE-CD substitution bands and to characterize the fingerprint of the composition (Fig. 10). Anion exchange chromatography (24) was utilized to isolate separate substitution bands (mono- to deca-derivatives) that were subsequently identified by NMR and fast atom bombardment mass spectroscopy (FAB-MS). 

G. Okafo, G. Harland and P. Camilleri, Enhanced resolution in the fingerprinting of short-chain oligonucleotides using the dodecasodium salt of phytic acid in capillary electrophoresis. Anal. 

Allard E, et al. Comparing capillary electrophoresis—mass spectrometry fingerprints of urine samples obtained after intake of coffee, tea, or water. Anal Chem 2008 80 8946-8955. 

Garcia-Perez I, et al. Metabolic fingerprinting with capillary electrophoresis. J Chromatogr A 2008 1204 130-139. 

Hu, S., Michels, D.A.,Fazal,M.A., Ratisoontom, C., Cunningham, M.L., Dovichi,NJ. (2004). Capillary sieving electrophoresis/micellarelectrokinetic capillary chromatography for two-dimensional protein fingerprinting of single mammalian cells. Anal. Chem. 76,4044-4049. 

The advantage of fluorescence-based assays is their high sensitivity. It is therefore perhaps surprising that few such systems have been developed for evaluating the enantioselectivity of enzyme-catalyzed reactions. Fluorescence as a detection method is used in an enzyme-coupled assay [26] (see Section 9.3.4.3) and in the capillary array electrophoresis [25] (see Section 9.3.6.5). If several substrates need to be screened simultaneously, fluorescence-based substrate arrays as enzyme fingerprinting tools can be used, although enantioselectivity still needs to be addressed [26e],... 

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