Capacity peak permeating volume

One of the most serious disadvantages of SEC is limited peak capacity that is, only a few separated bands can be accommodated within the total chromatogram, as all peaks must elute between the total exclusion volume and the total permeation volume. 

The peak capacity of SEC columns is relatively small separation occurs in the volume between the void volume and the total permeation volume of the column. Preferably, SEC should be used in conjunction with one of the other modes of HPLC. Table 3 lists membrane proteins analyzed by SEC and the buffers used for elution. In all cases, a detergent or compounds that prevent aggregation are present during chromatography, with SDS being the most popular. 

Size-exclusion chromatography (SEC) is a method in which molecules are separated by size due to differential permeation into a porous support. It requires complete solubility of the analytes in the mobile phase and elimination of all interactions with the bonded phase. In these respects, SEC is not as useful for the separation of peptides as it is for proteins because peptides vary drastically in solubility, charge, and hydrophobicity. Peak capacity in SEC is fairly low compared to other HPLC methods because all separations must occur in the internal volume (Vi) of the support, which is generally less than half the volume of mobile phase in the column. Despite these deficiencies, SEC can be very effective for separating peptides from dimers, aggregates, small molecules, proteins, and other molecules which differ by size. 

Size-exclusion chromatography is a method in which molecules are separated by size due to differential permeation into a porous support. This technique is especially useful for the separation of proteins because they are macromolecules frequently found in the presence of smaller and larger species. The peak capacity in SEC is fairly low compared to other HPLC methods because all separation must occur in the internal volume of the support,... 

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See also in sourсe #XX -- [ ]

See also in sourсe #XX -- [ ]

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