Capacity factors, chiral selectors

DK Lloyd, A Ahmed, F Pastore. A quantitative relationship between capacity factor and selector concentration in capillary electrophoresis and high-performance liquid chromatography evidence from the enantioselective resolution of benzoin using human serum albumin as chiral selector. Electrophoresis 18 958-964, 1997. 

Abou-Basha and Aboul-Enein [22] presented an isocratic and simple HPLC method for the direct resolution of the clenbuterol enantiomers. The method involved the use of a urea-type CSP made of hS )-indoline-2-carboxylic acid and (R)-1 -(naphthyl) ethylamine known as the Chirex 3022 column. The separation factor (a) obtained was 1.27 and the resolution factor (Rs) was 4.2 when using a mobile phase composed of hexane-1,2-dichloroethane-ethanol (80 10 10, v/v/v). The (+)-enantiomer eluted first with a capacity factor (k) of 2.67 followed by a (—)-enantiomer with a k of 3.38. Biesel et al. [23] resolved 1-benzylcyclohexane-1,2-diamine hydrochloride on a Chirex D-penicillamine column. Gasparrini et al. [24] synthesized a series of the chiral selectors based on /ra s -1,2 - d i a m i n o eye I o hexane. The developed CSPs were used for the chiral resolution of arylacetic acids, alcohols, sulfoxides, selenoxides, phosphinates, tertiary phosphine oxides, and benzodiazepines. In another study, the same authors [25] described the chiral resolution of /i-aminocstcrs enantiomers on synthetic CSPs based on a re-acidic derivatives of trans- 1,2-diaminocyclohexane... 

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