Calcium imaging

Figure 1.9, Intensity left) and calcium images (right) of Quin-2 fluorescence in COS cells.

J. R. Lakowicz, H. Szmacinski, and M. L. Johnson, Calcium imaging using fluorescence lifetimes and long-wavelength probes, /. Fluorescence 2(1), 47-62 (1992). 

Owens DF, Boyce LH, Davis MB, Kriegstein AR (1996) Excitatory GABA responses in embryonic and neonatal cortical slices demonstrated by gramicidin perforated-patch recordings and calcium imaging. J Neurosci 16 6414-23... 

Leinders-Zufall T., Greer C. A., Shepherd G. M. and Zufall F. (1998b) Visualizing odor detection in olfactory cilia by calcium imaging. Ann. N.Y. Acad. Sci. 855, 205-207. 

Wang J. W., Wong A. M., Flores J., Vosshall L. B. and Axel R. (2003) Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell 112, 271-282. 

Sachse S. and Galizia C. G. (2002) Role of inhibition for temporal and spatial odor representation in olfactory output neurons a calcium imaging study. J. Neurophysiol. 87(2), 1106-1117. 

Fig. 7 Biosynthesis of NATs and TRP channel activation by NATs. (a) Evidence for a fatty acyl CoA taurine A-acyltransferase activity was detected in mouse tissue by incubating taurine and arachidonoyl-CoA with various tissue lysates, (b) arachidonyl NAT was tested as an activator of the TRPV1 (black line), TRPV4 (gray line), and TRPM8 (dashed line) ion channels. Channel activation was measured using a Fura-2-based calcium-imaging assay, where the ratio between the fluorescence at 340 and 380 nm is reflective of cellular calcium concentrations...

The role of tau in synaptic dysfunction has not been as well documented as A. Tau has been reported to change intracellular calcium levels, probably via the interaction of extracellular tau with muscarinic receptors Ml and M3 (G6mez-Ramos et al., 2008). Also a spatially association of tau modifications with intraneuronal AP, in which both pathologies co-occur at synaspses has been reported (Takahashi et al., 2010). Finally, using two-photon calcium imaging in layer 2/3 of an AD mouse model cortical neurons, an increase in the frequency of spontaneous calcium transients in the vicinity of amyloid plaques was seen (Busche et al., 2008). 

Figure 14.14 Calcium imaging. (A) The fluorescence spectra of the calcium-binding dye Fura-2 can be used to measure available calcium ion concentrations in solution and in cells. (B) A series of images show Ca " spreading across a cell These images were obtained through the use of a fluorescent calcium-binding dye. The images are false colored red represents high Ca " ...

Mattheakis L, Ohler LD. Seeing the light Calcium imaging in cells for drug discovery. Drug Discovery Today HTS supplement 2002 1 15-19. 

In addition to the molecular and structural studies described above, several functional assays including extracellular and intracellular electrophysiological recordings, calcium imaging, and pharmacologi-... 

Figure 5.3 Tetraethylestercalix[4]arene and tetraphosphine oxide calixI4]arene (The tetra-ethylester cavity is selective for sodium while the larger tetraphosphine oxide cavity is selective for calcium. Images kindly provided by Prof Dermot Diamond, Dublin City University, Ireland.).

SKIRI, H. T., GALIZIA, C. G., MUSTAPARTA, H., Representation of primary plant odorants in the antennal lobe of the moth Heliothis virescens, using calcium imaging, Chem. Senses, 2004, 29, 253-267. 

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