Caco integrity marker

Augustijns P, Mols R (2004) E1PLC with programmed wavelength fluorescence detection for the simultaneous determination of marker compounds of integrity and P-gp functionality in the Caco-2 intestinal absorption model. J Pharm Biomed Anal 34 971-978. 

When using Caco-2 or TC7 cells, parameters such as transepithelial electrical resistance (TEER), differentiation marker, morphology, P-gp expression, and monolayer integrity need to be tested failure to do so is often the reason for wide interlaboratory variations. Caco-2 cell lines contain only a single cell type and do not form a mucus layer. As this can limit the absorption of lipophilic compounds in particular, the coculturing of Caco-2 cells and mucus-secreting lines such as HT29-MTX has been proposed to overcome this limitation [48]. 

---