C Quantitation of the Transferred Product and Diffusion

The technique of Western blotting is widely used and a lot of investigations have been undertaken in order to quantify protein recovery on the collecting membrane [143, 144, 145, 146, 147]. One of the most common difficulties related to the description of the DPD transfer process is the estimation of the yield of proteins transferred from the gel onto the collecting PVDF membrane. One of the solutions was to use radiolabeUed proteins [193]. 

Effect of the buffer (heterogeneous CAPS and homogeneous Vi Towbin)  

Effect of the electric field used for the transfer ImA/cm or square shape tension (SST)  

Comparison of the influence of the electric field on the protein recovery The efficiency of the transfer using standard transblotting techniques or adapted SST (used during the DPD process) was tested without the digestion step, the main parameters are shown in Table 9. 

For each of these experiments, 2 lanes of 1-DE mini-gel were used. One [ig of Bio-Rad M, standard was loaded on the first lane of the gel and 50 nCi of radiolabeUed proteins from Amersham-Pharmacia Biotech were loaded on the second lane. Proteins were separated using a standard technique [39], At the end of electrophoresis, the gel was washed for 3 minutes in de-ionised water, 1 minute in 

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