C-Fos immunoreactivity

Studies of sleep-active neuronal discharge across the sleep-wake cycle in freely moving animals provide important information about the hypnogenic process (see below) but, because of sampling limitations, are not suitable for systematic mapping of the exact locations of putative hypnogenic neurons. The application of the c-Fos immunoreactivity (IR) method to map sleep-active neurons has stimulated several advances. C-Fos IR is a marker of neuronal activation in most brain sites immunohistochemically labeled neurons can be mapped systematically. The localization of c-Fos IR following sustained sleep, but not... 

Wan, W. et ah, Differential induction of c-Fos immunoreactivity in hypothalamus and brain stem nuclei following central and peripheral administration of endotoxin, Brain Res. Bull., 32, 581, 1993. 

Until recently an involvement of neuronal HA in the hypothalamic regulation of oxytocin (OT) secretion was unknown. However, within the recent years it has been reported that HA administered centrally stimulates c-fos expression and c-fos immunoreactivity in oxytocinergic neurons and OT mRNA in the SON and PVN [21]. In accordance with this, central infusion of HA stimulates OT secretion, an effect which occurs via activation of HI and H2 receptors [28]. Although HA releases OT, this neuropeptide does not seem to be involved in the mediation of the HA-induced release of ACTH and PRL [28],... 

Lee MD, Somerville EM, Kennett GA, Dourish CT, Clifton PG. Tonic regulation of satiety by 5-HT receptors in the mouse converging evidence from behavioural and c-fos immunoreactivity studies Eur J Neurosci 2004 19(ll) 3017-3025. 

Young ST, Porrino LJ, Iadarola MJ (1991) Cocaine induces striatal c-Fos-immunoreactive proteins via dopaminergic D, receptors. Proc Natl Acad Sci USA 55 1291-1295. 

Heeb MM, Yahr P (1996) c-Fos immunoreactivity in the sexually dimorphic area of the hypothalamus and related brain regions of male gerbils after exposure to sex-related stimuli or performance of specific sexual behaviors. Neuroscience 72 1049-1071 Herbison AE (2006) Physiology of the gonadotropin-releasing hormone neuronal network. In Neill JD, Plant TM, Pfaff DW (eds) Knobil and Neill s physiology of reproduction. Elsevier, St Louis, pp 1415-1482... 

Systemic administration of an H3 receptor antagonist, ciproxifan (1 mg/kg in 0.2 ml, i.m.), induced c-fos expression in the vast majority of HA-immunoreactive... 

The immediate early gene product c-Fos (cellular feline osteosarcoma) has widely been used as a useful marker of neuronal activation. Fos, the protein encoded by the c-fos gene, is a transcription factor that triggers transcription in a cascade of cellular responses. To determine which neuron groups are involved in the response to PGD2 and/or adenosine, especially A2aR agonists, we examined Fos-immunoreactivity under these conditions (Scammell et al., 1998, 2001 Satoh et al., 1999). 

Wang H, Storlien LH, Huang XF. Influence of dietary fats on c-Fos-like immunoreactivity in mouse hypothalamus. Brain Res 1999 843 184—192. 

Liu, H.M. and Chen, H.H. (1994a) c-fos protein expression and ischemic changes in neurons vulnerable to ische-mia/hypoxia, correlated with basic fibroblast growth factor immunoreactivity. J. Neuropathol. Exp. Neurol. 53 598-605. 

Figure 1. Immunoreactivity produced by four different antibodies against c-Fos. (A) Antibody sc-253 gives intense staining in the vomeronasal receptor neurons in the basal half of the VNO. No immunoreactivity was seen in the receptor neurons after staining with (B) sc-52, (C) Ab-4, or (D) Ab-5.

Of the four commercial antibodies against c-Fos (sc-253, sc-52, Ab-4 and Ab-5) used to assess potential immunoreactivity in the unstimulated VNO of mice only sc-253 showed staining (Figure 1). The immunoreactivity from sc-253 was exclusively confined to the neuroepithelium of the VNO (Figure 2). Neither non-sensory epithelium or vomero-... 

Bressler, S.C. Baum, M.J. 1996. Sex comparison of neuronal Fos immunoreactivity in the rat vomeronasal projection circuit after chemosensory stimulation. Neuroscience, 71, 1063—1072. 

Pompeiano M, Cirelli C, Tononi G. Effects of sleep deprivation on fos-like immunoreactivity in the rat brain. Arch Ital Biol 1992 130 325-335. 

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