By-Layer Assembly of Proteins

Reports have shown that multi-layer thin films can be fabricated by an alternating deposition of two kinds of molecules with affinity to each other. This 

A surface coated with a SAM of 3-mercaptoproponic-acid, was used for the covalent attachment of Alexa-Fluor 647 labeled streptavidin (AF-SA) by means of NHS-active ester chemistry [38]. This reaction yielded an SPR minimum shift of A0 O.27° and contributed a fluorescence intensity of A/A 350 eps (layer b ). Subsequently, the alternate binding of biotinylated IgG and streptavidin from 20 pg ml-1 solutions was monitored. 

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