Burley, Stephen

TFIIA and TFIIB are two basal transcription factors that are involved in the nucleation stages of the preinitiation complex by binding to the TBP-TATA box complex. Crystal structures of the ternary complex TFIIA-TBP-TATA box have been determined by the groups of Paul Sigler, Yale University, and Timothy Richmond, ETH, Zurich, and that of the TFIIB-TBP-TATA box by Stephen Burley and collaborators. The TBP-DNA interactions and the distortions of the DNA structure are essentially the same in these ternary complexes as in the binary TBP-TATA complex. 

The following, in alphabetical order, have reviewed one or more chapters, correcting our errors of fact or interpretation and helping to ensure they have the appropriate balance and emphasis Tom Alber (University of California, Berkeley), Tom Blundell (Cambridge University, UK), Stephen Burley (Rockefeller University), Charles Craik (University of California, San Francisco), Ken Dill (University of California, San Francisco), Chris Dobson (Oxford University, UK), Anthony Fink (Unversity of California, Santa Cruz), Robert Fletterick (University of California, San Francisco), Richard Henderson (LMB, Cambridge, UK), Werner Kiihlbrandt (MPI, Frankfurt), David Parry (Massey University, New Zealand), Greg Petsko (Brandeis University), and David Trentham (NIMR, London, UK). 

Stephen R. Wasserman, David W. Smith, Kevin L. D Amico, John W. Koss, Laura L. Morisco, and Stephen K. Burley... 

Figure 28-13 (A) Stereoscopic ribbon drawing of the phyloge-netically conserved 180-residue C-terminal portion of the TATA-binding protein (TBP) from Arabidopsis thaliana. The sequence consists of two direct repeats, giving the protein an approximate twofold symmetry. From Nikolov et al.337 (B) Structure of the corresponding C-terminal core (residues 155-335) of the human TATA-binding protein (TBP) bound to the TATA sequence of a promoter in adenovirus DNA. From Nikolov et al.327 (C) Structure of human transcription factor IIB bound to a TBP from Arabidopsis thaliana, which, in turn, is bound to an adenovirus TATA sequence. Hypothetical B DNA extensions have been modeled at both ends of the DNA segment. The +1 at the left end is the transcription start site and the —43 upstream end is to the right. From Nikolov et al.338 Courtesy of Stephen K. Burley.

Stephen K. Burley, Steven C. Almo, Jeffrey B. Bonanno, Malcolm Capel, Mark R. Chance, Teiry Gaasterland, Dawei Lin, Andre] Sali, F. William Studier, Subramanyam Swaminathan, Structural genomics beyond the Human Genome Project, Nature Genetics, 23 (1999), 151-157. 

---