Buckwheat allergen detection

New trends in the analysis of food contaminants in general focus on the simultaneous detection of different analytes present in the same sample. A multiplex PCR assay has been developed for the simultaneous detection of wheat, buckwheat, and peanut (Hashimoto et al., 2007). A distinct amplicon size for each allergen can be observed in an agarose gel. Since some RT-PCR thermocyclers have the ability to detect several fluorescent probes, they can easily be adapted to detect several food allergens simultaneously. Moreover, other platforms commonly used for multiplexing can be used in combination with PCR. Microarray slides can be spotted with peptide nucleic acid (PNA) capture probes for PCR products obtained from the amplification of DNA... 

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