Bubble microtome

Finally mention should be made of a very different kind of surface layer sampler which has not yet been used for the study of organic film constituents. This is the bubble microtome as described by MacIntyre (1968). The jet drop released by a bubble bursting at a film-covered surface consists of material originally within a distance of 0.05% of the bubble dizimeter from the air/water interface. For 1-mm bubbles, for example, this represents a... 

MacIntyre, F., Bubbles a Boundary-layer Microtome for Micron-thick... 

Carrots treated with the dye-biopolymer complexes and processed under the optimum condition as determined by RSM were rehydrated and fixed for light microscopic examination. Thin slices of carrots (2-3 mm) were fixed on cork board and frozen in isopentane at -160°C for 15 sec. The frozen samples were warmed up to -20°C in a histostat cryostat microtome chamber and cut into sections of 28 micron thickness. The sections were fixed on slides with glycerol gel and dried in an oven at 35°C to remove any air bubble under the slides. The slides were examined and photographed under a light microscope at 500 and 785 times magnification. 

MacIntyre, F. (1968). Bubbles a boundary-layer Microtome for micron thick samples of liquid surface. J. Phys. Chem. 72, 589-592. 

---