Breakage models, protein

A limitation of both models is overestimation of the amount of protein released. Peptide predictions by the simple model are too high as well. The effect resembles a gap in the material balance, as if the models predict a larger quantity of proteinacious material than is actually present in the cells. Possibly some cytoplasmic proteins are not released during protoplast breakage. In figure 6, insoluble proteins could account for a substantial part of the residual yeast at 90 minutes digestion. 

It is known that ethylene imine and its derivatives allqrlate DNA and proteins (see review [1]). They induce mutations as it was shown in various model objects Drosophila, higher plants, fungi, bacteria, viruses and others) and breakage of the chromosome apparatus [2, 3]. Chemical compounds that cause mutations and breakage of chromosomes usually are called chemical mutagens. ... 

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