Brain slices acetylcholine synthesis

More recently Braganca, Faulkner, and Quastel (B15) showed that this inhibition of acetylcholine synthesis in brain slices by ammonia is consistent only in the diminution of bound acetylcholine. They further showed that the addition of inhibitors of glutamine synthesis, such as methionine sulfoxide, ethionine sulfoxide, and methionine sulfoximine (the toxic product, causing convulsions, formed in flour chemically aged with nitrogen trichloride) would partially reverse the ammonium inhibition. These observations were confirmed and extended to a wide variety of ATP-requiring reactions by Weil-Malherbe. In support of this suggestion was the observation that ammonia is taken up by the brain in hepatic coma (B8). The observations are valid and have been confirmed, but the interpretation of the data and the hypothesis are questionable. A quantitative basis for the evaluation of this mechanism can... 

B15. Braganca, B. J., Faulkner, P., and Quastel, J. H., Effects of inhibitors of glutamine synthesis on inhibition of acetylcholine synthesis in brain slices by ammonium ions. Biochim. et Biophys. Acta 10, 83 (1953). 

The action of melanocortins may involve cholinergic transmission. Acetylcholine synthesis in brain slices is increased by ACTH 1-39 and cur-... 

Turning from brain slices to subcellular fractions, the acetylcholine effect was localised in the synaptosomal fraction by Durell Sodd (1966). Synthesis of phosphatidic acid from [32p]ATP was sensitive to acetylcholine, but not synthesis from [ 2p] glycerophosphate. This indicated that reaction 2 rather than reaction 1 was involved (Hokin Hokin, 1959). Confirmation comes from the finding that diacylglycerol kinase is about ten times as... 

Effects of triethyllead acetate on the cholinergic system in the brain of the rats were investigated in vitro. At concentrations below 0.1 pM it inhibited the depolarized release of acetylcholine (ACh) from slices of cortex and the synthesis of ACh, while the non-depolarized release of ACh was potent in a dose-dependent manner135. 

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