Boiling beads

Sample Digestion. Samples (1-5 g or mL) are weighed or pipetted into 100 mL micro KJeldahl flasks containing 2 glass boiling beads. Depending on sample size, 3-5 mL of concentrated HNO3,... 

Elute the proteins by boiling beads in lx SDS sample buffer. 

Using boiling beads in the molten material to prevent large bubbles from forming. 

Spit (evaporation) A molten droplet of the evaporant ejected from the molten surface. Spits generally result from vapor bubbles rising through the molten material. See also Boiling beads. 

Note 1). The mixture is heated with a mantle with stirring for 32 hr under a 50-cm fractionating column packed with 5-nm glass beads and topped by a Dean-Stark trap. The reaction mixture is then distilled through the packed column. The fraction which boils at 120 -126°C is collected. The yield is 86.0-87.3 g (77-78%) of 2,2-dimethyl-4-pentenal (1) as a clear, colorless oil, n 1.4216 (Note 2). 

Bead Processes. These processes have generally replaced the above techniques. The styrene is polymerised by bead (suspension) polymerisation techniques. The blowing agent, typically 6% of low boiling petroleum ether fraction such as n-pentane, may be incorporated before polymerisation or used to impregnate the bead under heat and pressure in a post-polymerisation operation. 

The beads prepared above (11.58 g.) are suspended in 175 ml. of boiling absolute ethanol, and 0.94 g. (0.0159 mole) of aqueous 85% hydrazine monohydrate is added with stirring. The resulting mixture is refluxed for 10 hours, after which the polymer is collected by filtration and washed with 150-ml. portions of ethanol, aqueous 0.2A sodium hydroxide, distilled water, and anhydrous methanol. After vacuum drying at 60° for four hours, the yield of polymeric benzylamine is 10.38 g. 

Ethylene Dibromide. Technical material was washed three times with concentrated sulfuric acid, rinsed with water, and dried over calcium chloride. It was then distilled through 1 foot of glass beads. The fraction boiling between 130.2° and 131.2° C. was used. Every third day an aqueous solution was prepared by weighing approximately 0.5000 gram of ethylene dibromide into 2000.0 grams of water. It was stored in a dark bottle to minimize light decomposition. 

The immune complexes (beads) are washed 3 times with lysis buffer, and then 20 /il of 1 x SDS-PAGE sample buffer are added and the samples boiled for 5 min. The supernatant is subjected to SDS-PAGE, followed by western blotting. 

Pre-expansion a steam flow heats the beads of EPS, which are expanded to about 40 times their original size by the boiling of the pentane. Thus, a closed-cell foam is formed. 

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