Blumenkrantz method

Neutral sugars were quantified by trifluoroacetic acid hydrolysis (3) and gas chormatography of alditol acetates (4). Uronic acids were determined by Blumenkrantz method... 

Purity was confirmed by gel-filtration using a HPLC column packed with Asahipak GS-520HQ and elution with 100 mM sodium phosphate buffer containing 300 mM sodium chloride (pH 6.7). The content of total protein, total sugars, uronic acids, sulfates, nucleic acids, phosphate or fatty acids was assayed by the BCA [32] and Lowry method [33], the phenol-sulfuric acid method [34], the Blumenkrantz method [35], nephelometry [36], absorption at 260 nm, the Bartlett method [37] and the GLC method after methyl-esterification [38], respectively. 

Blumenkrantz, N. Asboe-Hansen G. 1973. New method for quantitative determination of uronic acids. Anal. Biochem. 54 484-489. 

Ramanathan, R., Su, A. D., Alvarez, N., Blumenkrantz, N., Chowdhury, S. K., Alton, K., and Patrick, J. (2000). Liquid chromatography/mass spectrometry methods for distinguishing N-oxides from hydroxylated compounds. Anal. Chem. 72 1352-1359. 

One of the most important reactions of hexuronic acids and glycuronans is the decarboxylation caused by treating with strong acids (usually 12% hydrochloric acid). The rapid and stoichiometric loss of 1 mol of carbon dioxide per mole has been developed as an analytical method by many workers.227 Methods based on the formation of colored phenolic compounds in strongly acidic media are widely used to assay total uronic acid.228,229 The method of Blumenkrantz and Asboe-Hausen228 has been adapted for microtiter plates.230... 

Electrophoresis Blow, D. M., see Holmes, K. C. Blumenkrantz, Nelly, and Asboe-Hanson, Gustav, Methods for Analysis of Connecdve-Tissue Macromolecules by Determination of Certain 13 1... 

Measurement of Total and Soluble Pectins. Total pectins In ethanol-insoluble powders were measured using the procedure described by Ahmed and Labavitch (31). Pectins were assayed colorimetrically using the method "o7 Blumenkrantz and Asboe-Hansen... 

The materials obtained are analysed for pectin content by uronlc acid assay. The method of Blumenkrantz and Asboe-Hansen (1973) Is employed. Further characterization of the pectlc material Included assay for neutral sugar composition by the alditol acetate method of Albershelm et al (1967), which entails derlvltlzatlon, and... 

Blumenkrantz, N., and G. Asboe-Hansen New Method for Quantitative Determination of Uronic Acids. Analyt. Biochem. 54, 484 (1973). 

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