1,3-Bisphosphoglycerate, from glyceraldehyde 3-phosphate

There would be an accumulation of those intermediates from glucose 6-phosphate to glyceraldehyde 3-phosphate and a depletion of those from 1,3-bisphosphoglycerate to pyruvate. 

Glyceraldehyde 3-phosphate is oxidized by NAD+, and inorganic phosphate (Pi) is incorporated into the product to form an acyl phosphate, 1,3-bisphosphoglycerate. NAD+ is reduced by transfer of a hydride ion (H ) from thiohemiacetal to the fourth position on the nicotinamide ring of NAD. ... 

Scheme 9.6. A cartoon representation of the enzyme (named glyceraldehyde-3-phosphate dehydrogenase, EC 1.2.1.12) catalyzed oxidation of the aldehyde glyceraldehyde-3-phosphate to the carboxylic acid-phosphoric acid mixed anhydride, 1,3-bisphosphoglycerate (which can be hydrolyzed to the corresponding carboxyhc acid and phosphoric acid). Start in a clockwise direction from the npper left.

Dekkera/Brettanomyces are unique with regard to carbohydrate utilization. Like other yeasts, Dekkera/Brettanomyces possess the glycolytic pathway and so will produce ethanol from acetaldehyde along with NAD, the latter being reused for the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate (Fig. 1.10). However, carbohydrates can also be oxidized to acetate (acetic acid) through acetaldehyde as follows ... 

Kvassman, J. Pettersson, G. (1989b). Mechanism of 1,3-bisphosphoglycerate transfer from phosphoglycerate kinase to glyceraldehyde-3-phosphate... 

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