Biphotonic microscopy imaging

Figure 5.3 (a) In vivo blood vessel biphotonic microscopy imaging of brain mouse, (b) 3D image of chromosomes during cell division by multiphotonic excitation of DAP ... 

Towards Biphotonic Microscopy Imaging 5.4.1 Proof of Concept... 

Microscopy imaging using Tb, Eu and even Yb containing probes has been reported and, in the case of the NIR emitter, the development of a new biphotonic microscopy set-up was achieved. 

However, to date, a real biphotonic luminescent lanthanide bio-probe suitable for biologically relevant microscopy imaging application remains to be discovered. Such probes must of course fulfill spectroscopic requirements like optimised two-photon brightness, but they have to present not only a good stability and solubility in biological media but also a functionalisation site for eventual bio-conjugation. One can anticipate that such an ideal lanthanide bio-probe will be prepared during the next decade [135]. 

Figure 5.7 Imaging of DNA by (a) mono and (b) biphotonic fluorescence microscopy of M5...

---