Biosynthesis of Glutamic Acid in C. glutamicum

Metabolic Regulation during Glutamic Acid Overproduction in C. glutamicum 11.3.2.1 Biosynthesis of Glutamic Acid in C. glutamicum 

GS NH3 -I- glutamic acid -1- ATP -s- glutamine + ADP + P GOGAT glutamine -1- 2-oxoglutarate -1- NADPH -1- H  

Moreover, the other biosynthetic pathway for glutamic acid is catalyzed by glutamate dehydrogenase (GDH) at high ammonia concentrations  

2 Relationship between Enzyme Activity of the 2-Oxoglutarate Dehydrogenase Complex and Glutamic Acid Production 

As described above, three enzymes are important for glutamic acid production ICDH, ODHC, and GDH. In particular, the activity of ODHC was thought to have an impact on glutamic acid production in C. glutamicum, as discussed in Section 11.4.2.1. ODHC consists of three subunits a catalytic subunit, Elo (OdhA) [52] a dihydrolipoamide acetyltransferase subunit, E2 (AceF) [53] and a dihydrolipoamide dehydrogenase subunit, E3 (Lpd) [54]. In addition, ODHC constitutes a supercomplex between the pyruvate dehydrogenase complex, which catalyzes the conversion from pyruvate to acetyl-CoA [53]. 

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