Dihydrolipoamide acetyltransferase

The second enzyme, dihydrolipoamide acetyltransferase [E2], shifts the acetyl residue from lipoamide to coenzyme A (2), with dihydrolipoamide being left over. 

As described above, three enzymes are important for glutamic acid production ICDH, ODHC, and GDH. In particular, the activity of ODHC was thought to have an impact on glutamic acid production in C. glutamicum, as discussed in Section 11.4.2.1. ODHC consists of three subunits a catalytic subunit, Elo (OdhA) [52] a dihydrolipoamide acetyltransferase subunit, E2 (AceF) [53] and a dihydrolipoamide dehydrogenase subunit, E3 (Lpd) [54]. In addition, ODHC constitutes a supercomplex between the pyruvate dehydrogenase complex, which catalyzes the conversion from pyruvate to acetyl-CoA [53]. 

PDHc comprises three enzymes and five coenzymes. The three primary enzymes include pyruvate dehydrogenase El component or pyruvate decarboxylase (El, EC 1.2.4.1), dihydrolipoamide acetyltransferase (E2, EC 2.3.1.12) and lipoamide dehydrogenase (E3, EC 1.8.1.4). El utilizes thiamin diphosphate (TPP) and Mg " as cofactors [7]. E2 contains covalently bound lipoyl groups [8], and E3 contains... 

Pyruvate dehydrogenase (lipoamide) [EC 1.2.4.1], which requires thiamin pyrophosphate, catalyzes the reaction of pyruvate with lipoamide to produce 5-acetyldihydroli-poamide and carbon dioxide. It is a component of the pyruvate dehydrogenase complex (which also includes dihydrolipoamide dehydrogenase [EC 1.8.1.4] and dihy-drolipoamide acetyltransferase [EC 2.3.1.12]). Pyruvate dehydrogenase (cytochrome) [EC 1.2.2.2] catalyzes the... 

---