Bioseparations chromatography

Manufacturing approaches for selected bioproducts of the new biotechnology impact product recovery and purification. The most prevalent bioseparations method is chromatography (qv). Thus the practical tools used to initiate scaleup of process Hquid chromatographic separations starting from a minimum amount of laboratory data are given. 

Keywords Preparative chromatography, Simulated moving bed chromatography, Continuous separation technique, Triangle theory, Bioseparation... 

At present, the purification by chromatographic processes is the most powerful high-resolution bioseparation technique for many different products from the laboratory to the industrial scale. In this context, continuous simulated moving bed (SMB) systems are of increasing interest for the purification of pharmaceuticals or specialty chemicals (racemic mixtures, proteins, organic acids, etc.).This is particularly due to the typical advantages of SMB-systems, such as reduction of solvent consumption, increase in productivity and purity obtained as well as in investment costs in comparison to conventional batch elution chromatography [1]. 

Muller, W. (1990). Liquid-liquid partition chromatography of biopolymers in aqueous two-phase systems. Bioseparation, 1(3—4), 265-282. 

Liquid column chromatography is the most commonly used method in bioseparation. As shown in Figure 11.6, the adsorbent particles are packed into a column as the stationary phase, and a fluid is continuously supplied to the column as the mobile phase. For separation, a small amount of solution containing several... 

Separation by Chromatography 175 Table 11.1 Liquid column chromatography used in bioseparation. 

Mattiasson, B., ed. Bioseparation International Journal of Separation Science. Special Issue Expanded Bed Chromatography. 8(1/5), The Netherlands Kluwer (1999). 

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