Biosensors ligand-receptor interactions

Biosensors for Proteins, Ligand-Receptor Interactions, and Enzyme Activities. 44... 

BLM systems have been accepted as models of natural biomembranes for applications in medicine, industry, and clinical laboratories. BLMs have therefore been studied extensively in combination with various proteins, and are an excellent choice for the basis for development of electrochemical biosensors. The principles behind the development of BLM-based biosensors are quite simple. The sensing element should be biocompatible and should have a structure similar to a biomembrane. Chemically selective proteins may then be embedded into the membranes with substantial retention of binding activity. The simplest way to test transducer function is by using ligand-receptor binding interactions... 

Figure 9.1 A general scheme of ligand-receptor contact interaction. The operation of an electrochemical biosensor which is based on the properties of a modified BLM or s-BLM. L, ligand (substrate, antigen, hormone, ion or electron, donor or acceptor) R, receptor (enzyme, antibody, receptor site, carrier or channel, acceptor or donor).

Hoffman, T. L., Canziani, G., Jia, L., Rucker, J., Dorns, R. W. (2000). A biosensor assay for studying ligand-membrane receptor interactions Binding of antibodies and HIV-1 Env to chemokine receptors. Proceedings of the National Academy of Sciences of the United... 

Figure 9 Illustration of the combined SPR-based BIA/MS approach (139). Deriva-tized biosensor chips, having multiple (2-4) flow cells each, are used in the real-time SPR-BIA analysis of interactions between surface-bound receptors and solution-phase ligands. The sensor chips are removed from the biosensor after SPR-BIA, with ligands still retained within the flow cells, and prepared for MALDI-TOF by application of an appropriate matrix to the flow cells. The matrix solution disrupts the receptor-ligand interaction, liberating the ligand into solution for incorporation into the matrix crystals. With proper application of the matrix, the crystals settle onto the original location of the interaction and spatial resolution between flow cells is preserved. The flow cells are targeted individually during MALDI-TOF and the retained ligand(s) are detected at precise and characteristic m/z values.

---