Bioreactors oxygen-limited growth

The medium for enzyme production was completely synthetic and limited in carbon to prevent excessive growth of mycelia. It contained glucose 2 g/l plus ammonia nitrogen, vitamins and trace elements according to Leisola et al.(ii). The 100 1 inoculum was transferred into 8001 of medium in a 10001 bioreactor equipped with 12 rrfi of nylon wool sheets supported by steel wire screen (Figure 1). The medium was continuously stirred with the impeller (100-200rpm) and saturated with pure oxygen. The temperature was maintained at 37°C. 

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