Biomolecular syntheses

In 1992, ACT replaced the Model 350 with the Model 396 Multiple Biomolecular Synthesizer. This instrument possesses two robotic arms, a variable speed orbital mixer, nitrogen assisted bottom filtration, a built-in ventilation system, protocols for both Fmoc and Boc synthesis and fully automated on-board cleavage for the Fmoc syntheses. It has reactor blocks of 8, 16, 40, and 96 wells for the synthesis of peptides from 5 pmol to 1 mmol. The Model 396 MBS also has a heater/cooler option for applications in solid-phase organic synthesis. 

ACT introduced the 357 Flexible Biomolecular Synthesizer in 1993.The Model 357 FBS is also a two arm robotic system, with a reaction block that incorporates thirty six 9.0-mL reaction wells and a single 600-mL well. The larger vessel permits the spht and mix technique for the generation of peptide combinatorial libraries.The scale range is 5 to 250 pmol and 9 mmol for the 9.0-mL and 600-mL reactor, respectively. The Model 357 FBS also has a variable speed orbital nnixer, nitrogen assisted bottom filtration and a built-in ventilation system. 

Hochfeld, W. L., Producing Biomolectdar Substances with Fermenters, Bioreactors, and Biomolecular Synthesizers, Chap. 9, CRC Press/Taylor Francis Group, Boca Raton, FL, 2006. 

Biomolecular Syntheses in Microreactors Peptide, Sugar and Oligosaccharide, and Oligonucleotide... 

This chapter presents a wide coverage of the state-of-the-art applications of microfluidic reactors for bioorganic and biocatalytic reactions. The development of microreactors for biomolecular syntheses although much slower compared to (iTAS is expected to continue to improve. Most biomolecules are expensive, and therefore high reaction yield and eflEdency are desired for their synthesis. The main challenge that remains for microreactor biomolecular syntheses is the integration of various components for chemical reactions such as synthesis, analysis, extraction, separation, and concentration. 

Microfluidic reactor for biomolecular synthesis Microreactor for synthesis of biomolecules Synthesis of biological molecules in microfluidics... 

Biomolecular Synthesis in Microfiuids, Fig. 1 (continued) the circuit. The various channels have been loaded with food dyes to help visualize the different components of the microfluidic chip colors are as in (a), plus green for... 

Biomolecular Synthesis in Microfluids, Fig. 4 (a) Silicon microfluidic microreactor, (b) Schematic of microreactor system, comprised of three primary inlets, a mixing and reaction zone, a secondary inlet for quench. 

Biomolecular Synthesis in Microfiuids, Fig. 5 Schematic of the microreactor for polyketide synthesis. (a) Biochip design used in this study, (b) Biosynthesis of flaviolin 4 and pyrone-based polyketides 2 and 3 from THNS with different starter CoA esters (Reproduced from Ku et al. [11])... 

Basically, biomolecular synthesis reaction in a microreaction system was performed by organic synthesis or biochemical conversion. The reaction was performed by simple loading of substrate and enzyme solutions into separate inlets using syringe pumps. 

Biomolecular Synthesis In Microfluids, Figure 1 (a) Schematic representation of a chemical reaction circuit used in the production of 2-deoxy-... 

Biomolecular Synthesis in Microfluids, Figure 2 Schematic of the microreactor used in carbodiimide coupiing reactions performed in ril (Reproduced from [1]) ... 

Biomolecular Synthesis in Microlluids, Rgute 6 Microreactor setup used in the reaction of amino acid with active ester [3]... 

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