Biomarkers peptide-based

Figure 7.10 Biomarker detection with an LC-MS chip. (A) Base-peak chromatogram of SCX fraction 7 of the MCF7 protein digest showing the distribution of putative biomarker peptides across the LC-MS run. (Adapted with permission from ref. 33). (B) Mass spectrum that enabled the selection of a PCNA peptide for collision-induced dissociation. Conditions were the same as for Figure 7.9.

Traditional biochemical techniques such as liquid chromatography (LC), gel electrophoresis, capillary electrophoresis (CE), and mass spectrometry (MS) have been widely used for the complete analysis of salivary proteins and peptides. The recent advances in these technical approaches applied to peptidomics have allowed a better comprehensive analysis of peptides in human whole saliva, envisioning the identification of potential salivary biomarkers of oral and systemic diseases. Sample preparation is a critical experimental step for the successful identification of peptides using MS-based approaches, for their quantitation and identification of PTMs. 

Over the recent years, proteomic technologies evolved rapidly and now offer new perspectives in discovery of new cancer biomarkers based on the detection of low molecular weight proteins or peptides by MS. Two main peptidomic approaches are currently under investigation. These include pattern recognition and single/oligo biomarker detection. 

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