Bioluminescence resonance energy transfer receptor

Cheng, Z. J. and Miller, L. J. (2001) Agonist-dependent dissociation of oligomeric complexes of G protein-coupled cholecystokinin receptors demonstrated in living cells using bioluminescence resonance energy transfer. J. Biol. Chem. 276,48040-48047. 

Mercier, J. F., Salahpour, A., Angers, S., Breit, A., and Bouvier, M. (2002) Quantitative assessment of beta 1 and beta 2-adrenergic receptor homo and hetero-dimerization by bioluminescence resonance energy transfer. J. Biol. Chem. 277,44925 14931. 

Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc. Natl. Acad. Sci. USA 97, 3684-3689. 

Alvarez-Curto E, Pediani JD, Milligan G (2010) Applications of fluorescence and bioluminescence resonance energy transfer to drug discovery at G protein coupled receptors. Anal Bioanal Chem 398 167-180... 

McVey M, Ramsay D, Kellett E, Rees S, Wilson S, et al. 2001. Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer. The human delta-opioid receptor displays constitutive oligomerization at the cell... 

Mercier JF, Salahpour A, Angers S, Breit A, Bouvier M. Quantitative assessment of pr and p2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer. J Biol Chem 2002 277 44,925 14,931. 

Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer... 

Angers, S., Salahpour, A., Joly, E., Hilairet, S., Chelsky, D., Dermis, M., et al. (2000). Detection of beta 2-adrenergic receptor dimerization in living cells rising bioluminescence resonance energy transfer (BRET). Proceedings of the National Academy of Sciences of the United... 

Lohse, M. J., Nuber, S., Hoffmann, C. (2012). Fluorescence/bioluminescence resonance energy transfer techniques to study G-protein-coupled receptor activation and signahng. Pharmacological Reviews, 64(2), 299—336. 

Bioluminescence resonance energy transfer (BRET) is a form of Eorster resonance energy transfer (RET). It is the non-radiative transfer of energy from an excited state donor to a ground state acceptor. The efficiency of energy transfer is highly dependent on the distance between the donor and acceptor moieties and their relative orientation with respect to each other. The photon emissions from the donor and receptor molecules during BRET reaction can be measured to quantify assay products. 

Trowell, S. C. Horne, I. M. Dacres, H. Leitch, V. Bioluminescent resonance energy transfer detection of compounds that bind or activate G protein-coupled receptors. PCX Int. Appl. WO 2010085844, 2010. 

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