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Biological macromolecules, purification affinity chromatography

Macromolecules such as proteins, polysaccharides, nucleic acids differ only in their physicochemical properties within the individual groups and their isolation on the basis of these differences is therefore difficult and time consuming. Considerable decreases may occur during their isolation procedure due to denaturation, cleavage, enz3rmatic hydrolysis, etc. The ability to bind other molecules reversibly is one of the most important properties of these molecules. The formation of specific and reversible complexes of biological macromolecules can serve as basis of their separation, purification and analysis by the affinity chromatography [6]. [Pg.60]

Affinity chromatography can be applied to the isolation and purification of virtually all biological macromolecules. It has been used to purify nucleic acids, enzymes, transport proteins, antibodies, hormone receptor proteins, drug-binding proteins, neurotransmitter proteins, and many others. [Pg.100]

Affinity chromatography is a special type of adsorption chromatography for the isolation and purification of biologically active macromolecules. It was used successfully for the first time in 1968 for the purification of enzymes [168]. Since then, innumerable proteins (e.g., enzymes. [Pg.316]


See other pages where Biological macromolecules, purification affinity chromatography is mentioned: [Pg.57]    [Pg.503]    [Pg.394]    [Pg.285]    [Pg.456]    [Pg.456]    [Pg.57]    [Pg.3]    [Pg.55]    [Pg.234]    [Pg.57]    [Pg.503]    [Pg.580]    [Pg.117]    [Pg.388]    [Pg.768]    [Pg.2613]    [Pg.2614]    [Pg.372]    [Pg.98]    [Pg.10]    [Pg.123]    [Pg.326]   
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