Biological examples of RET monitored by frequency-domain FLIM

5 Biological examples of RET monitored by frequency-domain FLIM 

RET in the last decade has been adopted from its sole usage in chemistry as a spectroscopic ruler to be applied in the life sciences as a biological ruler . To observe the physiological behaviour of signalling molecules it has been crucial to monitor their behaviour within the cellular environment without major perturbations. Today RET is used in microscopic imaging as a tool to measure in a precise manner inter- and intra-molecular distances in live or fixed cells. 

During cellular signal transduction, proteins continuously sense various membrane compartments and associate with specific proteins or lipids in response to localized physiochemical changes. Huorescence imaging methods have been utilized to quantify and localize protein-protein and protein-lipid associations in cells. 

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