Biochemical dissociated brain cells from

Biochemical, Morphological, and Regulatory Aspects of Myelination in Cultures of Dissociated Brain Cells from Embryonic Mice... 

L.L. Sarlieve, J.P. Delaunoy, A. Dierich, A. Ebel, M. Fabre, P. Mandel, G. Rebel, G. Vincendon, M. Wintzerith and A.N.K. Yusufi, Investigations on myelination in vitro. III. Ultrastruetural, biochemical, and immunohistochemical studies in cultures of dissociated brain cells from embryonic mice, J. Neurosci. Res. 6 659 (1981). 

Our initial studies were carried out on cultures of dissociated cells from brains of one day old mice. Unfortunately these preparations had lower than hoped for activities of certain biochemical parameters of myelination. For example, both the incorporation of 35g0jj into sulfolipids and the activity of the cyclic nucleotide phosphohydrolase attained relatively small activities even at the age (8 to 11 days) of optimum activity in culture (fig. 1 and 2). The dissociated cells from the 1 day old mouse produced parallel developmental patterns of both myelin parameters with peak activities at 8 to 11 days in culture. However cultures prepared from 15 day fetal brains proved to have relatively... 

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