Bioanalytical Systems thin-layer detector

In most cases the variable cell volume is of little practical use, since the minimum possible volume is usually required. The performance principles of detectors, where electrolysis takes place in the thin-layer of electrolyte, flowing parallel to the electrode surface, can be demonstrated on the commercially available thin-layer detector from Bioanalytical Systems (USA), schematically depicted in the Figure 12[36]. The detector works with a carbon paste or glassy carbon working electrode. The thickness of the layer is determined by the thickness of a Teflon film spacer ( 50 p) between two blocks of plastic. Since there is not enough space for location of the other two electrodes, they have to be placed outside of the thin-... 

The reaction coil is 20 cm of coiled stainless steel capillary tubing. The guard column (1.5 cm length, inside diameter) which protects the more expensive Cjg reversed-phase separating column (length = 30 cm, inside diameter = 3.9 mm) is packed with silica-based Cig material. Typical electrochemical detector cells which can be used are (i) Model TL-5 thin layer detector cell obtained from Bioanalytical Systems, (ii) Model EA 1096 wall jet detector cell obtained from Metrohm. 

Figure 27.5 Commercial thin-layer LCEC detectors. [Courtesy of Bioanalytical Systems, Inc.]...

Fig. 14.8 A thin-layer cell for use as a high pressure liquid chromatography electrochemical detector (courtesy of Bioanalytical Systems).

Detection electrochemical amperometric detector LC-4B (Bioanalytical Systems Inc.) mode single electrode cell geometry thin layer, 2 pm working electrode glassy carbon reference electrode silver / silver chloride range 10 nA. 

Amperometric detection included a BAS (Bioanalytical Systems, Inc.) LC-4B amperometric detector, a BAS TL-3 thin-layer amperometric flow cell (5 mil spacer), and an RC-2A reference compartment. The flow cell contained a carbon paste-paraffin oil working electrode, a silver/silver chloride reference electrode (RE-1), and a stainless steel auxiliary electrode. A BAS RYT strip-chart recorder was used. 

Detector E, Bioanalytical Systems Inc. TL-5 Kel-F glassy carbon thin-layer cell, LC-4 electronic controller, -1-0.78 V, 2-5 nAA ... 

FIGURE 8 Electrochemical detector cell (Bioanalytical Systems, Inc.), (a) Diagram of the flow cell. A = auxiliary electrode, W = working electrode. R = reference electrode, (b) Dual thin-layer working electrodes in parallel (1) and series (2) configurations. [From Bratin K., and Kissinger, P. T. (1981). J. Liq. Chromator. 4, 321-57. Reprinted with permission from Marcel Dekker, Inc.]... 

---