Affinity binding site

Figure 15.3 T3fpical heterogeneous distribution of binding site affinities found in molecular imprinted polymers compared with the homogenous distribution of a typical monoclonal antibody. Reprinted from Umpleby et al. (2001). Copyright 2001 American Chemical Society.

Kattelman, E., Venton, D. and LeBreton, G. (1986). Characterization of U46619 binding in unactivated, intact human platelets and determination of binding site affinities of four TXA2/PGH2 receptor antagonists (13-APA, BM 13.177, ONO 3708 and SQ 29,548) Thrombosis Res., 41, 471-481... 

Vasanth Kumar, K. Monteiro de Castro, M. Martinez-Escandell, M Molina-Sabio, M Rodriguez-Reinoso, F. (2010). A Continuous Binding Site Affinity Distribution Function from the Freundlich Isotherm for the Supercritical Adsorption of Hydrogen on Activated Carbon. Journal of Physical Chemistry C, 114,13759-13765. 

The Na,K ATPase exchanges intracellular sodium ions for extracellular potassium ions, the gastric ATPase H3O for extracellular potassium, and the sarcoplasmic or endoplasmic reticular Ca pump two cytoplasmic Ca for 2H in the sarcoplasmic reticulum. In the outward reaction transporting Na% H% or Ca % the catalytic subunit is phosphorylated by ATP, and in the inward reaction, the phosphoenzyme is dephosphorylated by K or H (in the case of the sarcoplasmic reticulum Ca ATPase). To achieve uphill transport at a significant rate, there has to be a decrease in binding-site affinity on the side of the membrane from which the ion is transported and reorientation of this site toward the side to which the ion is transported. 

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