Bifunctional alkylating drug

The immunostaining procedure described below can be applied, in principle, to the quantification of any stabilized drug/protein - DNA adduct or complex where antibodies are available for that adduct or complex. The protocol provided below describes the detection and measurement of the adducts formed between the bifunctional alkylating drug melphalan and DNA, where monoclonal antibodies have been elicited against the adducts (3). 

The drug Busulfan, although a bifunctional alkylating agent, is less reactive because it does not involve a strained ring intermediate. It nevertheless has been shown to cross-link guanine residues, at least in vitro. 

Several DNA binding drugs that act as bifunctional alkylating agents and are able to... 

Orally administered, narrow-range antibioiic used for Group A p-hemolytic streptococci. Bifunctional alkylating agent used as anti neoplastic drug against some lymphomas. 

Bartzatt, R., Suat, L., Cirillo, G., et al. Bifunctional constructs of aspirin and ibupro-fen (nonsteroidal anti-inflammatory drugs NSAIDs) that express antibacterial and alkylation activities. Biotechnol. Appl. Biochem. 37 273—282, 2001. 

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