Biosensor bienzymatic

ALP + bienzymatic biosensor (tyrosinase. CDH) Phenyl phosphate PhenoP Bienzyme catalytic biosensor with a Clark O2 electrode, 0.60 mV vs. Ag/AgCl E 3.2fM (320 zmole), T = 57 min 23... 

The Ag-Ab was collected in the effluent, the enzyme substrate PP added, and the product phenol detected at a bienzymatic biosensor identical to the one described earher. By packing the column with a large excess of cocaine, more than 200 assays were performed before the column required regeneration. 

Perez, S. and E. Fabregas. 2012. Amperometric bienzymatic biosensor for L-lactate analysis in wine and beer samples. Analyst 137(16) 3854-3861. 

Figure 3.27 Schematic representation of the construction of bienzymatic biosensor NaR-SODI-CNT-PPy-Pt electrode and illustration of reactions that take place during the simultaneous determination of N02 and NOs .

Table 3.6 Simultaneous Measurement of NO2" and NOb in Human Plasma Using the Bienzymatic Biosensor...

The stability of the bienzymatic NaR-SODl-CNT-PPy-Pt electrode was evaluated by monitoring the current response three times a day in the presence of 100 nM nitrite and 300 nM nitrate over four weeks, and the rest of the time it was stored at 4 °C. The SODl and NaR were quite stable, as inferred from their electrocatalytical activity of 92% and 89.6%, respectively, after one month of storage and 83% and 76%, respectively, after two months of storage. RepeatabiHty of the bienzymatic biosensor was tested by... 

Figure 3.41 Time versus current response of the bienzymatic biosensor obtained (a) after CA membrane coating and (b) before CA membrane coating upon addition of 250 pM AsA in 0.1 M PBS containing 100 nM N02 and 300 nM NOs at scan rate of 50 mV s versus Ag/AgCI.

Madasamy, T., Pandiaraj, M., Balamumgan, M., Bhargava, K., Sethy, N.K., Karunakaran, C., 2014. Copper, zinc superoxide dismutase and nitrate reductase coimmobilized bienzymatic biosensor for the simultaneous determination of nitrite and nitrate. Biosens. Bioelectron. 52, 209—215. 

A.A. Ciucu, C. Negulescu, and R.P. Baldwin, Detection of pesticides using an amperometric biosensor based on ferophthalocyanine chemically modified carbon paste electrode and immobilized bienzymatic system. Biosens. Bioelectron. 18, 303-310 (2003). 

M. Rochelet-Dequaire, N. Djellouli, B. Limoges, and P. Brossier, Bienzymatic-based electrochemical DNA biosensors a way to lower the detection limit of hybridization assays. Analyst 134(2), 349-353 (2009). 

Deepshikha etal. have fabricated a novel nanobiocomposite bienzymatic amperometric cholesterol biosensor, coupled with cholesterol oxidase (ChOx) and horseradish peroxidase (HRP), based on the gold nanoparticle-decorated graphene-nanostructured PANI nanocomposite (NSPANI-AuNP-GR) film which was electrochemically deposited onto indium tin oxide (ITO) electrode from the nanocomposite (NSPANI-AuNP-GR) dispersion, as synthesized by in situ polymerization technique [166]. The... 

Tian, R, and G. Zhu. 2002. Bienzymatic amperometric biosensor for glucose based on polypyrrole/ceramic carbon as electrode material. Anal Chim Acta 451 251. 

Zhu, L., et al. Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a caibon nanotubes electrode. Biosens. Bioelectron. 23(4), 528-535 (2007)... 

Cocaine He,E,N ALP/PP Bienzymatic recycling biosensor with EIA- immunocolumn 380 pM (38 fmole) 33... 

The electrochemical biosensor employed for the measurement of CySH is a bienzy-matic system comprising of SOD coimmobUized with horseradish peroxidase (HRP) (Dharmapandian et al., 2010). The bienzymatic cysteine biosensor was fabricated by the following procedure. 10 pL of SOD (0.2mgmL ) was first apphed onto the... 

---