Biantennary glycans

Table II Structures0 of Biantennary Glycans N-Glycosylically Linked to Protein... 

The synthesis of the 23-37 domain entailed coupling of a complex biantennary glycan to a masked peptide thioester, corresponding to residues 22-28 of epo, under conditions... 

Formation of the aminobenzamide derivative facilitated the structural determination of a monosialylated biantennary glycan found to be present on the serum transferrin of a patient with a new type of congenital disorder of glycosylation, CDG Ilh. In this case, the patient sample and appropriate standards were analyzed by negative ion nanospray tandem MS on a quadrupole orthogonal TOF instrument, and the single siahc acid residue could be clearly located at the terminus of the 6-arm (55). 

M. Dauchez, J. Mazurier, J. Montreuil, and G. Vergoten, Biochemie, 74, 63 (1992). Molecular Dynamics Simulations of a Monofucosylated Biantennary Glycan of the N-Acetyllac-tosamine Type The Human Lactotransferrin Glycan. 

CigHssNOis 503.456 Constit. of the anomalous biantennary glycan chain in human chorionic gonadotropin (hCG) isol. from the urine of a choriocarcinoma patient. 

Fig. 7. Conformations of (a) tetraantennary and (b) biantennary glycans. The amino acid residue asparagine (ASN) is at the bottom in both cases.

Fig. 8. The structures shown from above are (a) tetraantennaiy giycan. the diameter dl is 4.10 nm when calculated from the structure in (a) and 3.96 nm when calculated from die OWLS measurements (b) biantennary glycans, d2 is 4.80 nm from the structure in (b) and 4.78 nm from the OWLS measurements.

Fig. 4 Phytohemagglutin from Phaselous vulgaris (PHA-E) (a) interactions with biantennary glycan in two different manners determined by STD NMR analysis, (b) one biantennary glycan bridges two PHA-E molecules determined by STD NMR analysis.

The glycans on a recombinant IgG antibody, eg. plantibody , produced in transgenic tobacco plants have recently been compared with the carbohydrates on the complementary IgGl molecule, (Guy slS antibody) produced in a murine monoclonal cell line (Figure 8) [72] Four major biantennary glycan structures were... 

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