Bergmann glia

Barakat, L., Bordey, A. GAT-1 and reversible GABA transport in bergmann glia in slices. 

Rakic P, Sidman RL (1973) Weaver mutant mouse cerebellum defective neuronal migration secondary to abnormality of Bergmann glia. Proc Natl Acad Sci USA 70 240-244. 

Luque JM, Richards JG (1995) Expression of NMDA 2B receptor subunit mRNA in Bergmann glia. Glia 73 228-232. 

The highest concentrations of GLAST are seen in the molecular layer of the cerebellum, the Bergmann glia in particular (Lehre et ah, 1995). Compared to the cerebellum, the concentrations of GLAST protein in bulbus olfactorius, hippocampus, cerebral cortex and thalamus are 49, 35, 33 and 22%, respectively (Lehre et ah, 1995 Lehre and Danbolt, 1998 0. Haugeto and N.C. Danbolt, unpublished). 

The density of GLAST in the molecular layer is as high as 18,000 GLAST molecules per p.m- tissue (molecular layer) or about 4700 molecules per p.m Bergmann glia cell membrane (Lehre and Danbolt, 1998). The concentration of GLAST in the hippocampus (stratum radiatum, CAl) is 3200 molecules per p.m- tissue or about 2300 per p.m astroglial cell membrane. 

Fig. 59. 5 -Nucleotidase immunohistochemical staining of rat cerebellum. A. Immunofluorescence. B. PAP-method. Enzyme activity is predominantly found within the molecular layer on Bergmann glial fibers (long arrows). Purkinje cells are surrounded by fine rims of reaction product (small arrows). Within the granular layer 5 -nucleotidase activity is diffusely scattered between granule cells (arrow heads). Vibratome sections. C. Longitudinally sectioned Bergmann glia cell processes (B) of the molecular layer of rat cerebellum. Fine DAB reaction product is located on adjacent membranes of these processes (arrows). Bars in A,B = 50 /tm, in C = 0.5 nm. Schoen et al. (1987).

Symbols not detected +, positive ++, strongly positive PC, Purkinje cells GrC, granule cells BC, basket cells StC, stellate cells Bg, Bergmann glia DCN, deep cerebellar nuclei. 

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