Benzoylcholine chloride

Benzoylcholine chloride, 30,10 Benzoylcholine iodide, 30,10 Benzoylcholine picrate, 30, 13 Benzoyl-2-methoxy-4-nitroacetanilide, 37,4... 

Benzoylcholine chloride has been prepared by heating choline chloride with benzoyl chloride " and by the action of trimethyl-amine on 2-chloroethyl benzoate. ... 

Differentiation based on dibucaine inhibition. The most commonly used agent for differentiating serum cholinesterase variants is dibucaine, which acts as an inhibitor of the enzyme (Sections 2.2 and 6.2). Its use for the differentiation of cholinesterase variants was first introduced by Kalow and Genest (Kll) in 1957, and it has been and continues to be extensively used for this purpose. The assay is performed in two cuvettes, one with substrate alone and one with substrate plus dibucaine. Both cuvettes contain M/15 phosphate buffer (Sorensen) at pH 7.4 and 5 X 10 mol/liter benzoylcholine chloride. One of the cuvettes contains 1 X 10 mol/liter dibucaine. The initial velocity at 26°C is measured in both cuvettes by determining the rate of change of absorbance at 240 nm. The dibucaine number, DN, is the percentage of inhibition by dibucaine, and is calculated as DN = 100 (v - t> )/o, where V is the uninhibited velocity and v is the velocity measured in the presence of the inhibitor. DN values obtained by this method for the most widely studied cholinesterase variants are presented in Table 1 (see Section 2.1). 

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