Bathocuproine disulfonate

Dimethyl-l,10-phenanthrolinedisulfonic acid, di-Na salt (H2O) (bathocuproine-disulfonic acid di-Na salt) [52698-84-7] M 564.5, pKEst 0 (for free acid). Inorganic salts and some coloured species can be removed by dissolving the crude material in the minimum volume of water and precipitating by adding EtOH. Purified reagent can be obtained by careful evapn of the filtrate. 

In order to apply LRC to the setting of hydraulic mortars we selected the water-soluble rhenium complex Na2[(Bacu)Re(CO)3Cl] with Bacu = bathocuproine disulfonate as LRC-active probe [3]. This yellow salt was yet unknown but could be syntliesized by a simple procedure. This compound is characterized by a luminescence which consist of a single emission band which undergoes a blue shift from Xmax == 598 imi in aqueous solution to... 

Pyridylazo)resorcinol (PAR) 4-(2-Pyridylazo)resorcinol (PAR) + triethanolamine Bathocuproine disulfonic acid... 

Spec A = 470 nm to 550 nm of the Bathocuproine-disulfonic acid complex after extraction with chloroform and methanol by use of (PLS2)... 

We have recently found that the anti-melanoma activity of DSF is Cu dependent,(5P) and several lines of evidence suggest that the active species is Cu(deDTC)2. First of all, the anti-melanoma activity of DSF is enhanced by the addition of CuCla and attenuated in the presence of large excess of chelators, such as bathocuproine disulfonic acid, BCPD. Likewise, DSF treatment leads to significant increases in intracellular Cu concentrations, which can also be inhibited by BCPD. Chemical studies have shown that in aqueous media, Cu(II) salts react with DSF to directly form Cu(deDTC)2 in almost stoichiometric yields, via oxidative deconq)osition of small amounts of disulfiram. 

Abbreviations. Disulfiram (DSF), tetraethythiuram disulfide pDTC, pyrollidine- dithiocarbamate deDTC, diethyldithiocarbamate maltol, 2-methyl-3-hydroxy-4-pyrone BCPD, bathocuproine disulfonic acid. 

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