Basic Parameters Controlling Speed and Resolution

Other factors not covered by equation (17-23) are packing particle size and column temperature. These factors are also discussed below. These changes are discussed in more detail with examples in the resolution section. Keep in mind that these optimization approaches can be simulated in DryLab or similar software packages and should be used during the method optimization process. 

2 Resolution. Maximizing selectivity, as part of the early stages of methods development, will result in the fastest methods since as flow rate is increased or column length is decreased, resolution will decrease. The resolution equation describes the key parameters. In equation (17-24), k is substituted for the isocratic retention factor, k, to give 

TABLE 17-4. Influence of Gradient Parameters on Resolution, Retention, and Run Time 

33 Decrease Gradient Time (tg), Increase Flow Rate (F) Proportionally. 

5 Decrease Gradient Range (A%), Decrease Gradient Time (t ). The 

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