Based Screening Strategies

Output from biochemical high-throughput screenings is a list of active compounds with indicated activity values, e.g., ICjq. On a three-dimensional (3-D) level, relevant conformers of active molecules may be superimposed in order to locate similar 

B Hypothetical 4-point agrophore model based on four molecular 

Ligand-based approaches are generally used to accurately predict the activity of derivatives with optimized substitution pattern. In some cases, however. 

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Du, C., Zhang, Y., Li, Y. and Cao, Z. 2007a. Novel Redox Potential-Based Screening Strategy for Rapid Isolation of Klebsiella Pneumoniae Mutants with Enhanced 1,3-Propanediol-Producing Capability. Appl. Environ. Microbiol., 73, 4515—4521... 

Figure 11.13 Synthetic route to the Pt-Rh-O-Ir-Ru discrete catalyst library L9 and fluorescence-based screening strategy based on 11.8 to discover the novel catalysts 11.9 and 11.10.

Ashwin H, Stead S, Caldow M, Sharman M, Stark J, de Rijk A, Keely BJ, A rapid microbial inhibition-based screening strategy for fluoroquinolone and quinolone residues in foods of animal origin. Anal. Chim. Acta 2009 637(l-2) 241-246. 

These selectivity differences provide a rational basis for a three-column initial screening strategy a strongly hydrophobic column, a weakly hydrophobic column, and a phenyl column. Commercially available columns are not indexed in a manner that easily allows column selection based on hydrophobicity however, butyl columns are good candidates for strongly hydrophobic columns. Columns with names like ether-, iso-, and isopropyl are good candidates for weakly hydrophobic columns. 

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