Baddiley

After a full stmctural elucidation of coen2yme A ia 1953 by Baddiley, it became evident that pantothenic acid is one of the components of coen2yme A (2)... 

Characterised by conversion to the acridine salt by addition of alcoholic acridine (l.lg in 50mL), filtering off the yellow salt and recrystallising from H2O. The salt has m 215°(dec), A- ax 259nm (e 15,400) in H2O. [Baddiley and Todd J Chem Soc 648 1947, 582 1949, cf LePage Biochem Prep 1 1 I949 Martell and Schwarzenbach Helv Chim Acta 39 653 7956]. 

It has been recrystd from H2O (fine needles) and is freely soluble in boiling H2O. Crysts also from H2O by addition of acetone. Purified by chromatography on Dowex 1 (in formate form), eluting with 0.25M formic acid. It was then adsorbed onto charcoal (which had been boiled for 15min with M HCI, washed free of chloride and dried at 100°), and recovered by stirring three times with isoamyl alcohol/H20 (1 9 v/v). The aqueous layer from the combined extracts was evaporated to dryness under reduced pressure, and the product was crystallised twice from hot H2O. [Morrison and Doherty Biochem J19 433 7967]. It has A-max 259nm (e 15,400) in H2O at pH 7.0. [Alberty et al. J Biol Chem 193 425 7957 Martell and Schwarzenbach Heh Chim Acta 39 653 7956]. The acridinium salt has m 208° [Baddiley and Todd J Chem Soc 648 1947 Pettit Synthetic Nucleotides, van Nostrand-Reinhold, NY, Vol 1 252 1972 NMR Sarma et al. J Am Chem Soc 96 7337 1974 Norton et al. J Am Chem Soc 98 1007 1976 IR of diNa salt Miles Biochem Biophys Acta 27 324 1958],... 

Calam C.T. (1987) Process Development in Antibiotic Fermentations, Cambridge Studies in Biotechnology, 4 (eds Sir James Baddiley, N.H. Carey, J.F. Davidson, I.J. Higgins W.G. Potter). Cambridge Cambridge University Press. 

Brundish, D.E. and Baddiley, J. (1968) Pneumococcal C-substance, a ribitol teichoic acid containing choline phosphate. BiochemicalJournal 110, 573-582. 

Neuhaus FC, Baddiley J (2003) A continuum of anionic charge structures and functions of D-alanyl-teichoic acids in Gram-positive bacteria. Microbiol Mol Biol R 67 686-723... 

Powell DA, Duckworth M, Baddiley J (1975) Membrane-associated lipomannan in micrococci. Biochem J 151 387-397... 

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