Baculo viruses

Kobayashi M., Morita T., Ikeguchi K., Yoshizaki G., Suzuki T. and Watabe S. (2006) In vivo biological activity of recombinant goldfish gonadotropins produced by baculo-virus in silkworm larvae. Aquaculture. 256, 433-442. 

Lemer, C. G. and Saiki, A. Y. (1996) Scintillation proximity assay for human DNA topoisomerase I using recombinant biotinyl-fusion protein produced in baculo-virus-infected insect cells. Anal. Biochem. 240, 185-196. 

Wu J, Ruan Q, Peter Lam FfY (1998), Evaluation of spent medium recycle and nutrient feeding strategies for recombinant protein production in the insect cell-baculo-virus process, J. Biotechnol. 66 109-116. 

Szewczyk B, Floyos-Carvajal L, Paluszek M, Skrzecz I, Souza LM (2006), Baculo-viruses - re-emerging biopesticides, Biotechnol. Adv. 24 143-160. 

King, L.A., Mann, S.G., Lawrie, A.M. and Possee, R.D. (1994). Baculo-virus expression vector system Production and isolation of recombinant viruses. In Cell Biology A Laboratory Handbook, Vol. 3 (Celis, J.E., ed.), Academic Press, San Diego, pp. 148-154. 

Generating suitable protein is often the most labor intensive step on the way to 3D structures. Enough protein to create hundreds of crystals is needed and therefore care needs to be taken with expression and purification procedures. High yield expression and simple and effective purification protocols are beneficial. Optimized protein constructs for crystallization often lack glycosylation sites and carry affinity tags for purification. All standard structural biology protein expression systems are used to produce the proteins for fragment cocrystallization, i.e., E. coli, Baculo virus insect cell systems or mammalian cell lines. 

J.E. Vialard, M.A. Basil, C.D. Richardson Introduction to the molecular biology of baculo-viruses. In Methods in molecular biology, C. D. Richardson (Ed.). Humana Press 1995 203-224. Baculovirus expression protocols, vol. 39. 

Neame PJ, Barry FP. The link proteins. Experientia 1993 49 393 02. Grover J, Roughley PJ. The expression of functional link protein in a baculo-virus system analysis of mutants lacking the A, B and B domains. Biochem J 1994 300 317-324. 

The advent of contemporary biotechnology has enabled development of new strategies to achieve the same objectives protecting crops using biolr cally-produced, biodegradable pestiddes. A typical example is die production and use of proteinaceous insect toxins encoded by Baculo virus. Th fyp pestiddes are readitylnpgradable and are target spedfic. 

Because of the possible relevance of P450 4Z1 to cancer, we expressed the enzyme (baculo-virus system) in our own laboratory and screened a battery of carcinogens [350] for activation, but all were negative (Y. Xiao and F. P. Guengerich, unpublished results). 

Baculo virus Baculo virus E. coli E. coli nr... 

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