Bacterial pathogens decontamination

A filtered air supply should maintain a positive pressure relative to surrounding areas under ail operational conditions and flush the area effectively. Moreover, particular attention should be paid to the protection of the zone of greatest risk, that is, the immediate environment to which the product and the cleaned components in contact with it are exposed. The various recommendations regarding air supplies and pressure differentials may need to be modified if it becomes necessary to contain materials such as pathogenic, highly toxic, radioactive, or live viral or bacterial materials. Decontamination faalities and the treatment of air leaving a dean area may be necessary for some operations. 

At 70—140°C, peroxide is vaporised. Peroxide vapor has been reported to rapidly inactivate pathogenic bacteria, yeast, and bacterial spores in very low concentrations (133). Experiments using peroxide vapor for space decontamination of rooms and biologic safety cabinets hold promise (134). The use of peroxide vapor and a plasma generated by radio frequency energy releasing free radicals, ions, excited atoms, and excited molecules in a sterilising chamber has been patented (135). 

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