Bacteria genes sequences

Cluster Fx was also identified via its EPR spectral features in the RCI photosystem from green sulfur bacteria 31, 32) and the cluster binding motif was subsequently found in the gene sequence 34 ) of the (single) subunit of the homodimeric reaction center core (for a review, see 54, 55)). Whereas the same sequence motif is present in the RCI from heliobacteria (50), no EPR evidence for the presence of an iron-sulfur cluster related to Fx has been obtained. There are, however, indications from time-resolved optical spectroscopy for the involvement of an Fx-type center in electron transfer through the heliobacterial RC 56). 

Rasmussen, L.D., Zawadsky, C., Binnerup, S.J., Oregaard, G., Sprensen, S.J., and Kroer, N., Cultivation of hard-to-culture subsurface mercury-resistant bacteria and discovery of new merA gene sequences, Appl Environ Microbiol, 74 (12), 3795-3803, 2008. 

Neal RJ, Chater KF (1987) Nucleotide sequence analysis reveals similarities between proteins determining methylenomydn A resistance in Streptomyces and tetracycline resistance in eu-bacteria. Gene 58 229-241. 

To study and quantify microbial diversity one has to define what a species is. This is not a trivial objective since bacteria reproduce asexually and interbreeding cannot be applied for traditional species definition. While individual species of higher organisms may differ by as little as 1 % in their respective DNA, bacterial strains differ by up to 30%. Beside the so called species problem in microbiology, it is a widely accepted concept that bacteria with 16S rRNA gene sequences with similarity more than 98.7% (checked by DNA DNA hybridisation) belong to the same species. 

Clone propagation An extracted gene sequence can be inserted into a plasmid of bacteria. After clone propagation, the inserted gene sequences will be cut by restriction enzymes and isolated in useful yields via gel-electrophoresis. 

HoUibaugh, J. T., Bano, N., and Ducklow, H. W. (2002). Widespread distribution in polar oceans of a 16S rRNA gene sequence with affinity to Nitrosospira-]ike ammonia-oxidizing bacteria. Appl. Environ. Microbiol. 68, 1478—1484. 

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