Bacillus subtilis sporulation mutants

Bacillus subtilis 168 can be hcr or rec — Forward mutation sporulation mutants... 

E. coli K12 343/113 has been used for detection of forward mutation to resistance to 6-methyl tryptophan and loss of ability to ferment galactose.289 Forward mutation to sporulation mutants in Bacillus subtilis and to 8-... 

Despite the apparent connections between formation of antibiotics and spores, it has become clear that antibiotic production is not obligatory for spore formation [192]. The most damaging evidence to the antibiotic-spore obligatory hypothesis is the existence of mutants which form no antibiotic but still sporulate. Such mutants have been found in the cases of bacitracin (Bacillus licheniformis), myco-bacillin (Bacillus subtilis), linear gramicidin (B. brevis), tyrocidine (B. brevis), gramicidin S (B. brevis), oxytetracycline (Streptomyces rimosus), streptomycin (S. griseus), methylenomycin A (Streptomyces coelicolor), and patulin (Penicillium urticae). 

Critical amounts of cortex (a peptidoglycan layer of bacterial spores) are required for different properties of Bacillus sphaericus spores. Characteristic properties of the spores, such as the refractility and the resistance to xylene, octanol, and heat, were measured. Reduction with sodium borotritide and acid hydrolysis, etc., has been used to detect muramic lactam, which is located specifically in the cortical peptidoglycan of bacterial spores. The cortex content of several sporulation-deficient mutants of B. subtilis was examined using this procedure. 

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